Evaluation of chloroform/methanol and dichloromethane/hexane extractable lipids as surrogate measures of sample partition capacity for organochlorines in fish tissues

Organic contaminant concentration data are often lipid normalized to provide an indication of the chemical fugacity in the sample or to compare the relative equilibrium status of samples from the same environment. This study compared lipid normalized PCB and organochlorine concentration data for tis...

Full description

Saved in:
Bibliographic Details
Published in:Chemosphere (Oxford) 2004-04, Vol.55 (3), p.395-400
Main Authors: Drouillard, K.G., Hagen, H., Haffner, G.D.
Format: Article
Language:English
Subjects:
Adipose Tissue - chemistry
Agnatha. Pisces
Animal, plant and microbial ecology
Animals
Applied ecology
Biological and medical sciences
Chemistry Techniques, Analytical - methods
Chloroform
Ecotoxicology, biological effects of pollution
Effects of pollution and side effects of pesticides on vertebrates
Equilibrium partitioning
Esocidae
Fish lipids
Fugacity capacity
Fundamental and applied biological sciences. Psychology
Hexanes
Insecticides - analysis
Lipids - analysis
Liver - chemistry
Methanol
Methylene Chloride
Michigan
Muscles - chemistry
Ontario
PCBs
Polychlorinated Biphenyls - analysis
Techniques
Tissue distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Organic contaminant concentration data are often lipid normalized to provide an indication of the chemical fugacity in the sample or to compare the relative equilibrium status of samples from the same environment. This study compared lipid normalized PCB and organochlorine concentration data for tissues of individual fish when lipids were measured using chloroform/methanol (CM) or dichloromethane/hexane (DH) extractions. The CM extraction produced higher lipid yields for dorsal muscle (8-fold difference) and carcass (1.7-fold difference) compared to DH extractions, while both methods yielded comparable lipid contents for liver and adipose tissue. Lipid normalized tissue/carcass PCB and organochlorine concentration ratios were variable across tissue types when lipid contents from the CM technique were used, whereas, tissue/carcass concentration ratios approached the expected value of unity for each tissue when DH-derived lipids were used. These data suggest that the tissue lipid content as derived using the DH extraction provided a better surrogate measure of sample partition capacity than tissue lipids determined using the CM technique.
ISSN:0045-6535
1879-1298
DOI:10.1016/j.chemosphere.2003.11.010