New bacteriophage typing scheme for subdivision of the frequent capsular serotypes of Klebsiella ssp

A bacteriophage typing scheme for hospital isolates of Klebsiella spp. was developed. The scheme was designed specifically as a secondary typing method of discriminate between strains of serotypes K2, K3, and K21 but proved to be an efficient general typing method for strains of most serotypes. The...

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Bibliographic Details
Published in:Journal of clinical microbiology 1987-01, Vol.25 (7), p.1228-1232
Main Authors: Gaston, MA, Ayling-Smith, BA, Pitt, T L
Format: Article
Language:English
Subjects:
Klebsiella
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Summary:A bacteriophage typing scheme for hospital isolates of Klebsiella spp. was developed. The scheme was designed specifically as a secondary typing method of discriminate between strains of serotypes K2, K3, and K21 but proved to be an efficient general typing method for strains of most serotypes. The set of 15 phages gave 87.3% typeability on 236 strains of more than 70 different serotypes. Typeability within the K2, K3, and K21 strains was 93, 89, and 91%, respectively. There was a mean of 3.2 reactions strain super(-1) for all phage-typeable strains. Of the serologically nontypeable strains, 76.7% were susceptible to one or more phages. The most common pattern accounted for only 7% of the strains. A total of 96.5% of the strains were typeable by a combination of capsular serology and phage typing.
ISSN:0095-1137