Techniques in the shoot multiplication of the leguminous tree Prosopis alba clone B sub(2)V sub(50)

Tissue culture techniques involving shoot multiplication through axillary branching of the Prosopis alba clone B sub(2)V sub(50) are described, using single lateral bud explants from 1-2 y old, greenhouse-grown trees. Benzylaminopurine (BA) at concentrations of 0.0, 4.4 x 10 super(-8), 4.4 x 10 supe...

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Bibliographic Details
Main Authors: Tabone, T J, Felker, P, Bingham, R L, Reyes, I, Loughrey, S
Format: Conference Proceeding
Language:English
Subjects:
plant breeding
Prosopis alba
shoots
tissue culture
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Summary:Tissue culture techniques involving shoot multiplication through axillary branching of the Prosopis alba clone B sub(2)V sub(50) are described, using single lateral bud explants from 1-2 y old, greenhouse-grown trees. Benzylaminopurine (BA) at concentrations of 0.0, 4.4 x 10 super(-8), 4.4 x 10 super(-7), 4.4 x 10 super(-6), and 4.4 x 10 super(-5) M and indole-3-acetic acid (IAA) at concentrations of 0.0, 5.7 x 10 super(-9), 5.7 x 10 super(-8), 5.7 x 10 super(-7), 5.7 x 10 super(-6), and 2.9 x 10 super(-5) M showed significant interaction in the Murashige and Skoog (MS) basal salt medium. BA concentrations of 4.4 x 10 super(-5) or greater are apparently required for consistent shoot production. Up to seven multiple shoots and a maximum shoot length of 10 cm have been produced in 90 days on full-strength MS with 6.7 x 10 super(-5) BA and 2.9 x 10 super(-5) IAA. Ammonium (NH sub(4)) appears inhibitory to shoot development. Nitrate (NO sub(3)) and glutamine are acceptable nitrogen sources for short incubations (28 days), but after longer periods (55 days) shoots on media containing glutamine remain green and healthy, while those on nitrate media are yellow and senescent.
ISSN:0378-1127