Rapid determination of antimicrobial susceptibility patterns of Listeria monocytogenes isolates by the autobac AIS and MIC procedures

• Published in: Current microbiology 1987-01, Vol.15 (3), p.137-140
• Main Authors: DILLON, P. A, OVERMAN, S. B, OVERMAN, T. L
• Format: Article
• Language: English
• Subjects: Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Listeria monocytogenes; Microbiology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/BF01577261

A total of 34 isolates of Listeria monocytogenes were tested against ampicillin, cephalothin, chloramphenicol, erythromycin, tetracyclin and penicillin-G using the Autobac 3-h AIS and the Autobac 5-h MIC procedures. The results were compared to susceptibility category interpretations and MICs determined using the Sceptor system. With the Sceptor System, all isolates were interpreted to be moderately susceptible to ampicillin and penicillin-G, and susceptible to the four other antibiotics. With the Autobac AIS, all isolates were interpreted to be susceptible to all the antibiotics except penicillin-G. All but one of the 34 isolates were interpreted to be resistant to penicillin-G with the Autobac AIS test. The remaining isolate was interpreted to be indeterminant. The Autobac AIS test was unsatisfactory for determining the susceptibility of L. monocytogenes isolates to penicillin-G. The Autobac MIC results correlated well with the MIC results of the Sceptor system provided that the Autobac was programmed as though it were testing enterococci.