Cloning in E. coli of a streptomyces cellulase gene

A gene bank from Streptomyces A2, a cellulolytic actinomycete isolated from the gut of termites, has been constructed in the E. coli plasmid pAT153. The clones were screened for their capacity to degrade carboxymethylcellulose by the Congo red and cellulose azure techniques. A plasmid (pCSF1) showin...

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Bibliographic Details
Published in:Biotechnology letters 1987-07, Vol.9 (7), p.495-500
Main Authors: COPPOLECCHIA, R, DESSI, M. R, GIACOMINI, A, LEPIDI, A, MASTROMEI, G, NUTI, M. P, POLSINELLI, M
Format: Article
Language:English
Subjects:
Applied microbiology
Biological and medical sciences
Biotechnology
cellulase
cloning vectors
Escherichia coli
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
genes
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Microbiology
Miscellaneous
Molecular cloning
plasmids
Streptomyces
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Summary:A gene bank from Streptomyces A2, a cellulolytic actinomycete isolated from the gut of termites, has been constructed in the E. coli plasmid pAT153. The clones were screened for their capacity to degrade carboxymethylcellulose by the Congo red and cellulose azure techniques. A plasmid (pCSF1) showing cellulase activity in both tests was isolated and characterized. A restriction map of pCSF1 is reported.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF01027459