Genetic evidence that the outer membrane protein YOP1 of Yersinia enterocolitica mediates adherence and phagocytosis resistance to human epithelial cells

We studied the role of Yersinia enterocolitica outer membrane protein YOP1 in adherence and phagocytosis resistance to the human epithelial cell line, HEp‐2. A 5.2‐kb DNA fragment of the plasmid of Y. enterocolitica, serotype O : 9, which encodes a 225‐kDa outer membrane protein (YOP1), was cloned i...

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Published in:FEMS microbiology letters 1987-01, Vol.40 (1), p.37-41
Main Authors: Heesemann, Jürgen, Grüter, Ludwig
Format: Article
Language:English
Subjects:
Adhesion
Bacteriology
Biological and medical sciences
cell adhesion
epithelium
Fundamental and applied biological sciences. Psychology
man
Microbiology
Outer membrane protein
outer membranes
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
phagocytosis
Phagocytosis resistance
proteins
Yersinia enterocolitica
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creator Heesemann, Jürgen
Grüter, Ludwig
description We studied the role of Yersinia enterocolitica outer membrane protein YOP1 in adherence and phagocytosis resistance to the human epithelial cell line, HEp‐2. A 5.2‐kb DNA fragment of the plasmid of Y. enterocolitica, serotype O : 9, which encodes a 225‐kDa outer membrane protein (YOP1), was cloned into the mobilisable vector pRK290B. After introduction of the recombinant plasmid into a non‐adherent Y. enterocolitica strain of serotype O : 5, the resulting transconjugant acquired adherence and phagocytosis resistance properties to HEp‐2 cells. Furthermore, the plasmidless Y. enterocolitica strain of serotype O : 9 which is rapidly phagocytosed by HEp‐2 cells also became phagocytosis‐resistant after receipt of the YOP1‐encoding plasmid. These results indicate that YOP1 mediates cell adherence and phagocytosis resistance to HEp‐2 cells.
doi_str_mv 10.1111/j.1574-6968.1987.tb01978.x
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identifier ISSN: 0378-1097
ispartof FEMS microbiology letters, 1987-01, Vol.40 (1), p.37-41
issn 0378-1097
1574-6968
language eng
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source Alma/SFX Local Collection
subjects Adhesion
Bacteriology
Biological and medical sciences
cell adhesion
epithelium
Fundamental and applied biological sciences. Psychology
man
Microbiology
Outer membrane protein
outer membranes
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
phagocytosis
Phagocytosis resistance
proteins
Yersinia enterocolitica
title Genetic evidence that the outer membrane protein YOP1 of Yersinia enterocolitica mediates adherence and phagocytosis resistance to human epithelial cells
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