A Synthetic Peptide Vaccine Involving the Product of the Pre-S(2) Region of Hepatitis B Virus DNA: Protective Efficacy in Chimpanzees

The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the sequence of 19 amino acid residues (amino acids 14-32 from the N terminus) representing an area of high...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-12, Vol.83 (23), p.9174-9178
Main Authors: Itoh, Yukio, Takai, Emiko, Ohnuma, Hitoshi, Kitajima, Kohji, Tsuda, Fumio, Machida, Atsuhiko, Mishiro, Shunji, Nakamura, Tetsuo, Miyakawa, Yuzo, Mayumi, Makoto
Format: Article
Language:English
Subjects:
Amino acids
Animals
Antibodies
Antigens - immunology
Biological and medical sciences
Dosage
Fundamental and applied biological sciences. Psychology
Genes, Viral
Hepatitis antigens
Hepatitis B - prevention & control
Hepatitis B Surface Antigens - immunology
Hepatitis B vaccines
Hepatitis B virus
Hepatitis B virus - classification
Hepatitis B virus - genetics
Hepatitis B virus - immunology
Infections
Microbiology
Oligopeptides - immunology
Pan troglodytes
Species Specificity
Synthetic vaccines
Vaccination
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - immunology
Viral Proteins - immunology
Viral Vaccines - immunology
Virology
Yeasts
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Summary:The S gene encoding the major surface polypeptide of hepatitis B virus is preceded by the region pre-S(2) with a capacity to code for 55 amino acid residues. In the product of region pre-S(2), the sequence of 19 amino acid residues (amino acids 14-32 from the N terminus) representing an area of high local hydrophilicity is shared by viral strains of subtypes adr, ayw, and ayr; residue 22, phenylalanine, is replaced by leucine in a strain of the other subtype, adw. A synthetic peptide vaccine involving these 19 amino acid residues, when given to two chimpanzees, raised antibodies that bound to viral particles and protected the animals from challenge with 106 chimpanzee infectious doses of hepatitis B virus.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.23.9174