Adsorption of p,p'-Dichlorodiphenyldichloroethylene and Dieldrin in Largemouth Bass from a 60-d Slow-Release Pellet and Detection Using a Novel Enzyme-Linked Immunosorbent Assay Method for Blood Plasma

The adsorption of p,p'-DDE and dieldrin in Florida largemouth bass Micropterus salmoides floridanus was investigated using a 60-d slow-release pellet and a novel enzyme-linked immunosorbent assay (ELISA) method for blood plasma. The 60-d release pellets were inserted ip with a steel trochar, an...

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Bibliographic Details
Published in:Environmental toxicology and chemistry 2005-08, Vol.24 (8), p.1979-1979
Main Authors: Muller, Jennifer K, Sepulveda, Maria S, Borgert, Christopher J, Gross, Timothy S
Format: Article
Language:English
Online Access:Get full text
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Summary:The adsorption of p,p'-DDE and dieldrin in Florida largemouth bass Micropterus salmoides floridanus was investigated using a 60-d slow-release pellet and a novel enzyme-linked immunosorbent assay (ELISA) method for blood plasma. The 60-d release pellets were inserted ip with a steel trochar, and the DDE and dieldrin doses were 2.5, 5.0, and 10.0 mg/pellet and 0.25, 0.5, and 1.0 mg/pellet, respectively. High variability in DDE and dieldrin concentrations was observed in gonad, liver, muscle, and blood. Mean gonadal DDE concentrations correlated, however, with the amount of chemical purported to be contained in the pellets, whereas the gonadal dieldrin concentrations did not. Individual whole-blood concentrations varied in direct proportion to most tissue concentrations regardless of sex. For both blood DDE and dieldrin, the correlation between the ELISA and gas chromatography mass spectrometry methods were highly significant and linear.
ISSN:0730-7268