Astrocyte behavior and GFAP expression on Spirulina extract-incorporated PCL nanofiber

Nanomaterials are attractive for use in biological systems due to their ability to control the microenvironment of cells. Additionally, nanofibers can mimic fibrous characteristics of natural tissues. This study was conducted to assess astrocyte activity and infiltration behavior on Spirulina extrac...

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Published in:Journal of biomedical materials research. Part A 2013-12, Vol.101 (12), p.3467-3473
Main Authors: Min, Seul Ki, Kim, Cho Rong, Jung, Sang Myung, Shin, Hwa Sung
Format: Article
Language:English
Subjects:
Animals
astrocyte
Astrocytes - cytology
Astrocytes - drug effects
Astrocytes - metabolism
Astrocytes - ultrastructure
Biological and medical sciences
Blotting, Western
Cell Shape - drug effects
CNS
electrospun nanofiber
GFAP
Glial Fibrillary Acidic Protein - metabolism
Medical sciences
Nanofibers - chemistry
Nanofibers - ultrastructure
Polyesters - pharmacology
Rats
Rats, Sprague-Dawley
Spirulina
Spirulina - chemistry
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Technology. Biomaterials. Equipments
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Summary:Nanomaterials are attractive for use in biological systems due to their ability to control the microenvironment of cells. Additionally, nanofibers can mimic fibrous characteristics of natural tissues. This study was conducted to assess astrocyte activity and infiltration behavior on Spirulina extract‐embedded polycaprolactone (SP‐PCL) nanofiber. Astrocytes moved along with the nanofiber, and developed an elongated and stellate shape, which is similar to those in the natural neural tissue. In addition, the expression of GFAP, a biomarker representing the activation of astrocytes, was gradually up‐regulated with the increase of the concentration of Spirulina extract, indicating that Spirulina extract can control astrocyte activation. Overall, the results presented herein indicate that SP‐PCL nanofiber could be used in astrocyte tissue engineering for neuronal regeneration. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3467–3473, 2013.
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.34654