Synthesis and characterization of fluorescent dinucleotide substrates for the DNA-dependent RNA polymerase from Escherichia coli

New fluorescent derivatives of dinucleoside monophosphates, (5'-AmNS)UpA/ApU/Gpu/CpA, with a fluorophore, 1-aminonaphthalene-5-sulfonic acid (AmNS), attached to the first nucleotide of the dinucleoside monophosphates via a 5'-secondary amine linkage were synthesized in good yield. The abil...

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-08, Vol.262 (22), p.10684-10688
Main Authors: TYAGI, S. C, WU, F. Y.-H
Format: Article
Language:English
Subjects:
Biological and medical sciences
Escherichia coli
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Transcription. Transcription factor. Splicing. Rna processing
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Summary:New fluorescent derivatives of dinucleoside monophosphates, (5'-AmNS)UpA/ApU/Gpu/CpA, with a fluorophore, 1-aminonaphthalene-5-sulfonic acid (AmNS), attached to the first nucleotide of the dinucleoside monophosphates via a 5'-secondary amine linkage were synthesized in good yield. The ability of analogs to be incorporated into the 5' terminus of RNA chain forming fluorescent oligonucleotides by Escherichia coli RNA polymerase was studied in the presence of a synthetic DNA template.
ISSN:0021-9258
1083-351X