Expression of ricin A chain in Escherichia coli

DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P N25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A c...

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Bibliographic Details
Published in:FEBS letters 1987-05, Vol.216 (1), p.73-78
Main Authors: O'Hare, Mary, Roberts, Lynne M., Thorpe, Philip E., Watson, Graham J., Prior, Bernadette, Lord, J.Michael
Format: Article
Language:English
Subjects:
Biological and medical sciences
cDNA
cloning
DNA - genetics
E.coli
Escherichia coli
Escherichia coli - metabolism
Expression
Fundamental and applied biological sciences. Psychology
Gene expression
Genetic Vectors
Humans
Immunotoxin
Molecular and cellular biology
Molecular genetics
Operator Regions, Genetic
plasmids
Promoter Regions, Genetic
Protein Biosynthesis - drug effects
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - pharmacology
Recombinant Proteins - biosynthesis
Ricin - biosynthesis
Ricin - genetics
Ricin - pharmacology
ricin A
Ricin A chain
Ricinus communis
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Summary:DNA encoding ricin A chain was derived from preproricin cDNA and ligated into the expression vector pDS5/3. Transcription is controlled from the coliphage promoter P N25 fused with the lac operator of E.coli. When induced, E.coli 71.18 cells transformed with the recombinant plasmid express ricin A chain which is soluble and has full biological activity.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80759-7