EPR redox study of cytochrome c sub(3) from Desulfovibrio vulgaris) Miyazaki

The authors report the results of an EPR potentiometric titration of cytochrome c sub(3) from Desulfovibrio vulgaris) Miyazaki: the EPR spectral features of the four hemes are identified. The four midpoint redox potentials, which are deduced from the integrated intensity variations as a function of...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1987-01, Vol.217 (1), p.57-61
Main Authors: Gayda, J P, Yagi, T, Benosman, H, Bertrand, P
Format: Article
Language:English
Subjects:
cytochrome c3
E.S.R
potentiometric titration
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The authors report the results of an EPR potentiometric titration of cytochrome c sub(3) from Desulfovibrio vulgaris) Miyazaki: the EPR spectral features of the four hemes are identified. The four midpoint redox potentials, which are deduced from the integrated intensity variations as a function of the redox potential, are within the range -230 to -360 mV with two nearly equal intermediate values, in agreement with previous electrochemical measurements. A structural change of the environment of the heme with the most negative potential is observed during the first step of the reduction. The correspondence between the redox sites as characterized by EPR potentiometric titration, and the hemes in the tridimensional structure, is discussed.
ISSN:0014-5793