Crystallization of 70 S ribosomes and 30 S ribosomal subunits from Thermus thermophilus

Well-ordered three-dimensional crystals of 70 S ribosomes and 30 S ribosomal subunits from extremely thermophilic bacteria Thermus thermophilus have been obtained. Positively stained thin sections of the crystals have been analyzed by electron microscopy. Redissolved crystalline ribosomes and small...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1987-08, Vol.220 (2), p.319-322
Main Authors: Trakhanov, S.D., Yusupov, M.M., Agalarov, S.C., Garber, M.B., Ryazantsev, S.N., Tischenko, S.V., Shirokov, V.A.
Format: Article
Language:English
Subjects:
30 S Ribosomal subunit
Biological and medical sciences
Cell structures and functions
Electron microscopy
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Ribosome
Thermus thermophilus
Three-dimensional crystal
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Well-ordered three-dimensional crystals of 70 S ribosomes and 30 S ribosomal subunits from extremely thermophilic bacteria Thermus thermophilus have been obtained. Positively stained thin sections of the crystals have been analyzed by electron microscopy. Redissolved crystalline ribosomes and small ribosomal subunits reveal sedimentation constants of 70 S and 30 S, respectively, and are functionally active in the poly(U)-system.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80838-4