Validation and comparison of the Copan Milk Test and Delvotest SP-NT for the detection of antimicrobials in milk

The Delvotest SP-NT and Copan Milk Test, two microbiological tests designed for screening antimicrobial substances in milk were compared and validated. The performance criteria described by the European Decision 2002/657/EC were used for the study. Both tests were evaluated with visual and automated...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2007-03, Vol.586 (1-2), p.280-283
Main Authors: Le Breton, Marie-Hélène, Savoy-Perroud, Marie-Claude, Diserens, Jean-Marc
Format: Article
Language:English
Subjects:
Analytical chemistry
Animals
Anti-Bacterial Agents - analysis
Anti-Bacterial Agents - chemistry
Anti-Infective Agents - analysis
Anti-Infective Agents - chemistry
Antibiotics
Cattle
Chemistry
Chemistry Techniques, Analytical - instrumentation
Chemistry Techniques, Analytical - methods
Drug Residues - analysis
Exact sciences and technology
Food Analysis - instrumentation
Food Analysis - methods
Inhibitory test
Milk
Milk - metabolism
Reproducibility of Results
Research Design
Screening
Sensitivity and Specificity
Validation
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The Delvotest SP-NT and Copan Milk Test, two microbiological tests designed for screening antimicrobial substances in milk were compared and validated. The performance criteria described by the European Decision 2002/657/EC were used for the study. Both tests were evaluated with visual and automated reading (scanner) and the validation was performed on 10 different antibiotics (penicillin-G, cloxacillin, sulfamethazine, sulfadiazine, oxytetracycline, gentamicin, cephalexin, cefquinome, dihydrostreptomycin and trimethoprim). Both tests were found to detect penicillin, cloxacillin, sulfamethazine, sulfadiazine, cephalexin and gentamicin at or below the EU maximum residue limits (MRLs). Some other antibiotics such as oxytetracycline, dihydrostreptomycin, trimethoprim and cefquinome were not detected or only with a low sensitivity. Both tests were found easy to use, robust and fulfilled EU requirements.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2006.11.060