Evolutionary conservation of the 3' ends of members of a family of giant secretory protein genes in Chironomus pallidivittatus

Two cDNA clones representing the 3'-end regions of BR1 and BR2 75S mRNA were obtained from Chironomus pallidivittatus . The regular structure characterizing the core of these genes, consisting of tandemly arranged repeat units, changes into a more irregular structure toward the 3' end. Dis...

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Bibliographic Details
Published in:Journal of molecular evolution 1987-01, Vol.25 (1), p.20-28
Main Authors: Saiga, H, Grond, C, Schmidt, E R, Edstroem, J-E
Format: Article
Language:English
Subjects:
Chironomidae
Chironomus pallidivittatus
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Summary:Two cDNA clones representing the 3'-end regions of BR1 and BR2 75S mRNA were obtained from Chironomus pallidivittatus . The regular structure characterizing the core of these genes, consisting of tandemly arranged repeat units, changes into a more irregular structure toward the 3' end. Distal to a standard type of repeat unit with a characteristic excess of positive charges, a new type of repeat with a high, negative charge density is interspersed among parts of the standard unit. Analysis of the level of silent site divergence shows that the homology increases in the 3' direction up to the polyadenylation site. That the unique region is retained as a part of the secreted protein is shown by Western blotting.
ISSN:0022-2844
DOI:10.1007/BF02100037