Toxin resistance and/or male fertility reversion is correlated with defined transcription changes in the 1.5kb AvaI region of cmsT

Reversion of T type cytoplasmic males sterility (cmsT) to fertility is correlated with sequence changes in a 1.5kb AvaI fragment. This 1.5kb AvaI fragment is composed of 5' flanking sequences of ATPase subunit 6, one complete open reading frame (ORF 13) and part of the another (ORF 25). The seq...

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Bibliographic Details
Published in:Nucleic acids research 1987-08, Vol.15 (15), p.6091-6103
Main Authors: JI QIN, FAURON, C. M.-R, BRETTELL, R. I. S, MILHOUS, M, ABBOTT, A. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Gene expression
Molecular and cellular biology
Molecular genetics
Zea mays
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Summary:Reversion of T type cytoplasmic males sterility (cmsT) to fertility is correlated with sequence changes in a 1.5kb AvaI fragment. This 1.5kb AvaI fragment is composed of 5' flanking sequences of ATPase subunit 6, one complete open reading frame (ORF 13) and part of the another (ORF 25). The sequence of the 1.5kb AvaI fragment was compared to the sequences of homologous regions in the N(male fertile) and T revertant V sub(3) mitochondrial DNA. To further characterize the transcription of these rearranged sequences, specific probes for ORF 13, ORF 25 and 5' flanking sequences of ATPase 6 were hybridized to Northern blots of N, cmsT and the T revertant V sub(3) and V sub(18) mtRNAs. Each revertant has a single ORF 25 homologous transcript in contrast to the multitranscript pattern in cmsT. ORF 13 homologous transcripts were not detected in either revertant cytoplasm.
ISSN:0305-1048
1362-4962
DOI:10.1093/nar/15.15.6091