DNA typing of HLA-DR sub( beta ) chain genes can discriminate between undetected alleles and real homozygotes

The polymorphism of HLA-DR antigens has been studied by Southern blot hybridization under conditions specific for the detection of the DR sub( beta ) chain genes. Haplotype-specific patterns were defined with DNA from DR1, 2, 3, 4, 7, w8, w11, w12, and W13 homozygous typing cells, with restriction e...

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Bibliographic Details
Published in:Immunogenetics (New York) 1987-01, Vol.26 (4-5), p.249-257
Main Authors: de Preval, C, Angelini, G, Boogh, B, Ferrara, G B, Mach, B
Format: Article
Language:English
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Summary:The polymorphism of HLA-DR antigens has been studied by Southern blot hybridization under conditions specific for the detection of the DR sub( beta ) chain genes. Haplotype-specific patterns were defined with DNA from DR1, 2, 3, 4, 7, w8, w11, w12, and W13 homozygous typing cells, with restriction enzymes Eco RI, Bg1 I, and Pvu II. Certain serological specificities, such as DR2, DR3, and DR7, can be encoded by distinct allelic forms of DR sub( beta ) chain genes. The procedure of "DNA typing" was applied to family analysis of individuals expressing only a single DR specificity upon serological typing.
ISSN:0093-7711