Novel method for screening pigeonpea varieties for globulin content by radioimmunoassay

A rapid and sensitive radioimmunoassay (RIA) is described for screening a large number of pigeonpea varieties for quantitation of globulin content. This method employs precipitation of iodine-labelled pigeonpea globulin by polyethylene glycol and measuring the labelled antigen-antibody complex. RIA...

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Bibliographic Details
Published in:Biotechnology techniques 1988-03, Vol.2 (1), p.53-56
Main Authors: Mawal, M.R, Mawal, Y.R, Guru Row, T.N, Ranjekar, P.K
Format: Article
Language:English
Subjects:
Cajanus cajan
cultivars
globulins
protein content
radioimmunoassays
screening
seed development
seeds
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Summary:A rapid and sensitive radioimmunoassay (RIA) is described for screening a large number of pigeonpea varieties for quantitation of globulin content. This method employs precipitation of iodine-labelled pigeonpea globulin by polyethylene glycol and measuring the labelled antigen-antibody complex. RIA when used to quantitate the pigeonpea globulin levels during post anthesis revealed that maximum globulin is present between 24 to 28 days after flowering.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF01874208