Effects of diallyl sulfide and zinc on testicular steroidogenesis in cadmium-treated male rats

• Published in: Journal of biochemical and molecular toxicology 2008-09, Vol.22 (5), p.345-353
• Main Author: Sadik, Nermin A. H.
• Format: Article
• Language: English
• Subjects: Allyl Compounds - administration & dosage; Allyl Compounds - pharmacology; Animals; Antioxidants - metabolism; Biomarkers - metabolism; Body Weight - drug effects; Cadmium; Cadmium - administration & dosage; Cadmium - toxicity; Diallyl Sulfide; Follicle Stimulating Hormone - blood; Luteinizing Hormone - blood; Male; Marker Enzymes; Organ Size - drug effects; Rats; Rats, Wistar; Steroids - biosynthesis; Sulfides - administration & dosage; Sulfides - pharmacology; Testis; Testis - anatomy & histology; Testis - drug effects; Testis - enzymology; Testis - metabolism; Testosterone; Testosterone - blood; Zinc; Zinc - administration & dosage; Zinc - pharmacology
• ISSN: 1095-6670; 1099-0461
• DOI: 10.1002/jbt.20247

Cadmium (Cd) is one of the environmental pollutants that affect various tissues and organs including testis. Harmful effect of cadmium on testis is known to be germ cell degeneration and impairment of testicular steroidogenesis. In the present study, the effect of diallyl sulfide (DAS), a sulfur‐containing volatile compound present in garlic, and zinc (Zn) was investigated on cadmium‐induced testicular toxicity in rats. Male adult Wistar rats treated with cadmium (2.5 mg/kg body wt, five times a week for 4 weeks) showed decreased body weight, paired testicular weight, relative testicular weight, serum testosterone, luteinizing hormone, follicle‐stimulating hormone, and testicular total antioxidant capacity (TAC) and protein levels. Testicular steroidogenic enzymes, such as 3β‐hydroxysteroid dehydrogenase (3β‐HSD) and 17β‐hydroxysteroid dehydrogenase (17β‐HSD), and marker enzymes, such as sorbitol dehydrogenase (SDH), lactate dehydrogenase (LDH), acid phosphatase (ACP), alkaline phosphatase (ALP), and glucose‐6‐phosphate dehydrogenase (G6PD), showed a significant decrease in activities whereas that of γ‐glutamyl transferase was significantly increased after cadmium exposure. The results have revealed that concurrent treatment with DAS or zinc restored key steroidogenic enzymes, SDH, LDH, and G6PD and increased testicular weight significantly. DAS restored the TAC level and increased testosterone level and relative testicular weight significantly. Zinc restored testicular protein level and body weight. It can be concluded that cadmium causes testicular toxicity and inhibits androgen production in adult male rats probably by affecting pituitary gonadotrophins and that concurrent administration of DAS or zinc provides protection against cadmium‐induced testicular toxicity. © 2008 Wiley Periodicals, Inc. J Biochem Mol Toxicol 22:345–353, 2008; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20247