Diversity of Vicia faba circular mtDNA in whole plants and suspension cultures

A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences....

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Bibliographic Details
Published in:Theoretical and applied genetics 1986-07, Vol.72 (4), p.541-547
Main Authors: Negruk, V.I, Eisner, G.I, Redichkina, T.D, Dumanskaya, N.N, Cherny, D.I, Alexandrov, A.A, Shemyakin, M.F, Butenko, R.G
Format: Article
Language:English
Subjects:
Biological and medical sciences
culture media
DNA
Fundamental and applied biological sciences. Psychology
Genes. Genome
genome
mitochondria
Molecular and cellular biology
Molecular genetics
suspensions
Vicia faba
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Summary:A comparative analysis of the Vicia faba mitochondrial genome in whole plants and in longterm suspension culture has been conducted. Restriction fragment patterns of the mtDNA isolated from these two sources were notably different. Electronmicroscopic analysis also revealed significant differences. Large circular mtDNA patterns shifted from a 37-80 kb subpopulation, which was predominant in whole plants, to 18-34 kb subpopulations although in both classes notable quantities of circular molecules of 80 to 120 kb and more were also found. Both in whole plant and suspension culture cells very large circular DNAs were observed. Some of them had lengths nearly 290 kb and could be considered as evidence of the existence of master chromosomes. The minicircular DNA population was also altered. In the suspension culture we observed a notable increase of percentage of minicircles with sizes near 1 kb. Simultaneously, the percentage of minicircles with sizes near 3.5-10 kb significantly increased in suspension culture cells. In addition, a new peak (10-12 kb) of minicircles appeared. Copy number alterations for some sequences homologous to CCC1A, CCC1B and CCC2 (Negruk et al. 1982, 1985) were shown. Southern hybridization revealed the existence of a family of minicircles having sizes 1.4-2 kb with predominance of CCC1A, CCC1B and CCC2. The copy numbers of CCC1B and some minor minicircles was changed in the suspension culture when compared with the whole plants.
ISSN:0040-5752
1432-2242
DOI:10.1007/BF00289538