Tetrahymena pyriformis cells are deficient in all mannose-P-dolichol-dependent mannosyltransferases but not in mannose-P-dolichol synthesis

Cells of the ciliated protozoan Tetrahymena pyriformis incubated with ( super(14)C)glucose were found to synthesize Man-P-dolichol and Glc-P-dolichol, as well as Glc sub(3)Man sub(3)GlcNAc sub(2)-P-P-dolichol, the latter being the main and largest lipid derivative formed. The missing mannose residue...

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Bibliographic Details
Published in:Biochemistry (Easton) 1987-09, Vol.26 (18), p.5937-5943
Main Authors: Yagodnik, Claudia, De la Canal, Laura, Parodi, Armando J
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Carbohydrates
Fundamental and applied biological sciences. Psychology
Other biological molecules
Tetrahymena pyriformis
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Summary:Cells of the ciliated protozoan Tetrahymena pyriformis incubated with ( super(14)C)glucose were found to synthesize Man-P-dolichol and Glc-P-dolichol, as well as Glc sub(3)Man sub(3)GlcNAc sub(2)-P-P-dolichol, the latter being the main and largest lipid derivative formed. The missing mannose residues were those known to be transferred from Man-P-dolichol in other systems. Formation of Man-P-dolichol and of dolichol-P-P-oligosaccharides containing up to 5 mannose units was detected in cell-free assays containing protozoan membranes, rat liver dolichol-P, unlabeled Man sub(4-9)GlcNAc sub(2)-P-P-dolichol from pig liver, and GDP-( super(14)C)Man. Under exactly the same conditions but with UDP-( super(14)C)Glc instead of GDP-(14)Man, Glc-P-dolichol and dolichol-P-P-oligosaccharides containing 5 mannose and 2 to 3 glucose residues were formed in the absence of the pig liver compounds.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00392a054