Metallothionein levels in Algerian mice (Mus spretus) exposed to elemental pollution: An ecophysiological approach

• Published in: Chemosphere (Oxford) 2008-04, Vol.71 (7), p.1340-1347
• Main Authors: Marques, Carla Cristina, Gabriel, Sofia Isabel, Pinheiro, Teresa, Viegas-Crespo, Ana Maria, Mathias, Maria da Luz, Bebianno, Maria João
• Format: Article
• Language: English
• Subjects: Abandoned mines; Animal, plant and microbial ecology; Animals; Applied ecology; Biological and medical sciences; Biomarkers - analysis; Body Weight - drug effects; Ecosystem; Ecotoxicology, biological effects of pollution; Elemental contamination; Environmental Monitoring - methods; Fundamental and applied biological sciences. Psychology; General aspects; Kidney - metabolism; Liver - metabolism; Mammalia; Metallothionein; Metallothionein - analysis; Metals, Heavy - analysis; Metals, Heavy - toxicity; Mice - metabolism; Mice - physiology; Mining; Mus spretus; Organ Size - drug effects; Portugal; Seasons; Soil Pollutants - analysis; Soil Pollutants - toxicity; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/j.chemosphere.2007.11.024

The potential use of metallothioneins (MTs) as biomarkers of trace metal contamination was evaluated for the first time in the Algerian mouse (Mus spretus). Mice were collected seasonally in an abandoned mining area (Aljustrel) and in a reference area, both located in southern Portugal. MT levels were quantified in liver and kidney by differential pulse polarography and hepatic elemental concentrations (Mn, Fe, Cu, Zn, Se) were determined by particle-induced X-ray emission. Hepatic iron and selenium concentrations were elevated in mice from Aljustrel mine when compared to reference animals. MTs levels were averagely higher in mice from Aljustrel than those originated from the reference area. A season-dependent significant effect was found on the hepatic and renal MT concentrations, characterized by higher levels in winter and lower in autumn. In contaminated mice positive relationship between liver elemental contents (Cu in autumn and Fe in winter) and MTs were found. The seasonal variation of MT suggests that probably physiological and environmental factors could influence hepatic and renal MT induction. Results seem to imply that some environmental disturbance occur in the vicinity of the Aljustrel mine. Therefore, for the management purposes MT levels should be followed in liver of M. spretus, especially in winter. Furthermore, other physiological factors that could influence MT expression and turnover in Algerian mouse should also be monitored.