Whole‐cell ELISA for typing Neisseria meningitidis with monoclonal antibodies

Large‐scale screening of Neisseria meningitidis strains is necessary for epidemiological studies as well as for identifying immunologically important antigens. We have developed a new and simpler type of ELISA for this purpose. Whole bacteria from the strains being studied are coated onto PVC plates...

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Bibliographic Details
Published in:FEMS microbiology letters 1987-12, Vol.48 (3), p.367-371
Main Authors: Abdillahi, Hussein, Poolman, Jan T.
Format: Article
Language:English
Subjects:
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Monoclonal antibodies
Neisseria meningitidis
Serotyping
Subtyping
Whole‐cell ELISA
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Summary:Large‐scale screening of Neisseria meningitidis strains is necessary for epidemiological studies as well as for identifying immunologically important antigens. We have developed a new and simpler type of ELISA for this purpose. Whole bacteria from the strains being studied are coated onto PVC plates; the type and subtype are then determined by the binding of monoclonal antibodies with known specificities, detected with a protein A‐conjugate. This technique is rapid and easy but still sensitive and reproducible and is thus highly suitable for screening for antigens in a relatively native state on large numbers of strains.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1987.tb02626.x