Identification of a repeated sequence in the genome of the sea urchin which is transcribed by RNA polymerase III and contain the features of a retroposon

A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Stronglylocentrotus purpuratus has been characterized, and shown to contain the sequence feat...

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Bibliographic Details
Published in:Nucleic acids research 1988-01, Vol.16 (4), p.1431-1452
Main Authors: Nisson, P E, Hickey, R J, Boshar, M F, Crain, WR Jr
Format: Article
Language:English
Subjects:
Strongylocentrotus purpuratus
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Summary:A repeated sequence element which is located about 200 nucleotides upstream from the protein-coding portion of the muscle actin gene (probably within a large 5' intron) in the genome of the sea urchin, Stronglylocentrotus purpuratus has been characterized, and shown to contain the sequence features which indicate that it has been transposed by means of an RNA intermediate. This retroposon-like sequence, SURF1-1, is a member of a family which is dispersed and repeated about 800 times in the genome, referred to as SURF1 (sea urchin retroposon family 1). In vitro transcription of this sequence by RNA polymerase III defines a 300 nucleotide transcription unit which is bounded by a short direct repeated sequence.
ISSN:0305-1048