Complete amino acid sequence of poplar plastocyanin b

A second type of plastocyanin, plastocyanin b (PC b) was isolated from leaves of poplar Populus nigra var. Italica. The complete amino acid sequence of 99 residues in the single polypeptide chain of plastocyanin b has been determined: V DVLLGADDG SLAFVPSEFS VPA GEKIVFK NNAGFPHN VL FDED AV PSGV D V S...

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Bibliographic Details
Published in:FEBS letters 1987-12, Vol.226 (1), p.17-22
Main Authors: Dimitrov, Mitko I., Egorov, Caesii A., Donchev, Anthony A., Atanasov, Boris P.
Format: Article
Language:English
Subjects:
amino acid sequence
Analytical, structural and metabolic biochemistry
Biological and medical sciences
ChT
chymotryptic peptides of PCb
CNBr cleavage peptides of PCb
Fundamental and applied biological sciences. Psychology
Metalloproteins
Molecular heterogeneity
Other metalloproteins
Plastocyanin a
Plastocyanin b
Populus nigra
Primary structure
Proteins
tryptic peptides of PCb
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Summary:A second type of plastocyanin, plastocyanin b (PC b) was isolated from leaves of poplar Populus nigra var. Italica. The complete amino acid sequence of 99 residues in the single polypeptide chain of plastocyanin b has been determined: V DVLLGADDG SLAFVPSEFS VPA GEKIVFK NNAGFPHN VL FDED AV PSGV D V SKISMSEE DLLNAKGETF EVALS D KGEY T FYCSPHQGA GMVGKV I VN The sequence obviously demonstrates, that PC b, in comparison with the known plastocyanin, plastocyanin a (PC a), has 12 amino acid replacements (underlined letters): Ile 1→Val, Ile 21-Ser 22-Pro 23→Val-Pro-Ala, Ile 39-Val 40→Val-Leu, Ser 45-Ile 46→ Ala-Val, Ala 52→Val, Asn 76→Asp, Ser 81→Thr and Thr 97→Ile. The replacements at positions 40 and 76 of PC b are probably essential for differences in its redox and electrochemical properties, respectively.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80542-2