Both VP2 and VP3 are synthesized from each of the alternatively spliced late 19S RNA species of simian virus 40

The late 19S RNAs of simian virus 40 consist of a family of alternatively spliced RNAs, each of which contains open reading frames corresponding to all three of the virion proteins. Two approaches were used to test the hypothesis that each alternatively spliced 19S RNA species is translated to synth...

Full description

Saved in:
Bibliographic Details
Published in:Journal of virology 1988, Vol.62 (3), p.944-953
Main Authors: GOOD, P. J, WELCH, R. C, BARKAN, A, SOMASEKHAR, M. B, MERTZ, J. E
Format: Article
Language:English
Subjects:
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Microbiology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Virology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The late 19S RNAs of simian virus 40 consist of a family of alternatively spliced RNAs, each of which contains open reading frames corresponding to all three of the virion proteins. Two approaches were used to test the hypothesis that each alternatively spliced 19S RNA species is translated to synthesize preferentially only one of the virion proteins. First, the authors analyzed the synthesis of virion proteins in simian virus 40 mutant-infected monkey cells that accumulate predominantly either only one spliced 19S RNA species or only the 19S RNAs. Second, the authors determined the virion proteins synthesized in a rabbit reticulocyte lysate programmed with specific, in vitro-transcribed 19S RNA species. These results indicated that VP2 and Vp3, but not VP1, are synthesized from all 19S RNA species.
ISSN:0022-538X
1098-5514
DOI:10.1128/jvi.62.3.944-953.1988