Utilization of pyruvate in Lactobacillus casei subsp. rhamnosus ATCC 7469

The characteristics of pyruvate utilization in Lactobacillus casei subsp. rhamnosus ATCC 7469, grown in synthetic medium with 33 mM glucose and 100 mM pyruvate, were investigated. This Lactobacillus utilizes a transport system that depends on the presence of glucose and presents a saturation kinetic...

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Bibliographic Details
Published in:Current microbiology 1987-01, Vol.15 (5), p.259-264
Main Authors: BENITO DE CARDENAS, I. L, VALENTIN LEDESMA, O, OLIVER, G
Format: Article
Language:English
Subjects:
Bacteriology
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Growth, nutrition, cell differenciation
Lactobacillus casei
Microbiology
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Summary:The characteristics of pyruvate utilization in Lactobacillus casei subsp. rhamnosus ATCC 7469, grown in synthetic medium with 33 mM glucose and 100 mM pyruvate, were investigated. This Lactobacillus utilizes a transport system that depends on the presence of glucose and presents a saturation kinetics of 33 mM Km and a Vmax of 1 mu mol/5 min/mg dry weight. It has an optimum pH of 6.0 and an optimum temperature of 37 degree C. It is present in a synthetic medium without pyruvate, so that it is constitutive. It is not inhibited by glucose when this is added to the synthetic growth medium at a concentration of 1 g%. It is specific for pyruvate.
ISSN:0343-8651
1432-0991
DOI:10.1007/BF01589377