Uptake of auxotrophic cells of a heterocyst forming cyanobacterium by tobacco protoplasts, and the fate of their associations

Auxotrophic cells of the filamentous cyanobacterium Anabaena variabilis Kütz. were introduced into protoplasts of Nicotiana tabacum L. in an attempt to engineer a nitrogen-fixing endosymbiosis. Conditions were established to maximize uptake of the cyanobacteria by use of polyethylene glycol. Culture...

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Bibliographic Details
Published in:Planta 1978, Vol.139 (1), p.55-60
Main Authors: Meeks, J.C, Malmberg, R.L, Wolk, C.P
Format: Article
Language:English
Subjects:
Anabaena variabilis
Cell culture techniques
Cell lines
Chemical suspensions
Cultured cells
Cyanobacteria
Liquids
Plant cells
Plants
Protoplast culture
Protoplasts
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Summary:Auxotrophic cells of the filamentous cyanobacterium Anabaena variabilis Kütz. were introduced into protoplasts of Nicotiana tabacum L. in an attempt to engineer a nitrogen-fixing endosymbiosis. Conditions were established to maximize uptake of the cyanobacteria by use of polyethylene glycol. Culture of the novel association was not successful: tobacco protoplasts with Anabaena inside did not appear to divide. Most of the protoplasts expelled the cyanobacteria and simultaneously disintegrated. The reasons for the incompatibility are not known.
ISSN:0032-0935
1432-2048
DOI:10.1007/BF00390810