Effects of antiproteolytic agents on corneal epithelial viability and matrix metalloproteinase-2 and metalloproteinase-9 activity in alkali-burned corneas of rats

Objective To evaluate the effects of agents on corneal re‐epithelization and metalloproteinase‐2 and metalloproteinase‐9 (MMP‐2 and MMP‐9) activities in corneas of rats submitted to ulceration. Animals Studied Ninety eight healthy rats. Procedures Corneal ulcers were created using 1N NaOH in their l...

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Published in:Veterinary ophthalmology 2014-01, Vol.17 (1), p.23-31
Main Authors: Trujillo Piso, Dunia Y., Ribeiro, Alexandre P., Silva, Miguel L., Guimarães, Patrícia J., Morales, Adriana, Martins, Bianca C., Padua, Ivan M., Renzo, Roberta, Andrade, Alexandre L., Uscátegui, Ricardo R., Laus, José L.
Format: Article
Language:English
Subjects:
Acetylcysteine - therapeutic use
Animals
Caustics - toxicity
Chondroitin Sulfates - therapeutic use
cornea
Corneal Diseases - chemically induced
Corneal Diseases - drug therapy
Epithelium, Corneal - drug effects
Epithelium, Corneal - injuries
Female
Free Radical Scavengers - therapeutic use
Gene Expression Regulation, Enzymologic - drug effects
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinase 9 - metabolism
metalloproteinases
Proteolysis - drug effects
rat
Rats
Rats, Wistar
Sodium Hydroxide - toxicity
ulcerative keratitis
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Summary:Objective To evaluate the effects of agents on corneal re‐epithelization and metalloproteinase‐2 and metalloproteinase‐9 (MMP‐2 and MMP‐9) activities in corneas of rats submitted to ulceration. Animals Studied Ninety eight healthy rats. Procedures Corneal ulcers were created using 1N NaOH in their left eye. Eyes were treated every 6 h with 1% ethylenediaminetetraacetic acid (EDTA), 3% chondroitin sulfate (CS), 10% N‐acetylcysteine NAc and saline (S) at 6‐h intervals. Corneas were stained with fluorescein and photographed at the same time points. Following 20 h and 40–42 h of corneal injury, corneas were processed for scanning electron microscopy (SEM) to quantify microvilli density, and MMPs activities were analyzed using zymography. Results The percentage of wound area and the time in hours for corneal re‐epithelization did not differ significantly among treatment groups (P > 0.05). In first and the second moments, latent MMP‐2 was significantly elevated in the eyes treated with NAC and CS (P  0.05); significantly higher activity was observed in the second moment in the eyes treated with CS (P
ISSN:1463-5216
1463-5224
DOI:10.1111/vop.12032