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Increased endocytosis of fluorescent phospholipid in tobacco pollen in microgravity and inhibition by verapamil

Gravity sensing in plants occurs in specialised tissues, like in the columella in root tips or the endodermis in shoots. Generally, dense organelles, acting as statoliths, are thought to interact with the cytosekeleton and ion channels in gravitropism. We examined the possibility that tobacco pollen...

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Published in:	Plant biology (Stuttgart, Germany) Germany), 2014-01, Vol.16 (s1), p.107-112
Main Authors:	Scherer, G. F. E., Quader, H.
Format:	Article
Language:	English
Subjects:	Boron Compounds - metabolism Endocytosis Endocytosis - drug effects Fluorescence Heterocyclic Compounds, 3-Ring - metabolism hypergravity microgravity Nicotiana - drug effects Nicotiana - growth & development Nicotiana - metabolism Nicotiana - physiology phospholipid Phospholipids - metabolism Pollen - drug effects Pollen - metabolism tobacco pollen Verapamil - pharmacology Weightlessness
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container_end_page	112
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container_title	Plant biology (Stuttgart, Germany)
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creator	Scherer, G. F. E. Quader, H.
description	Gravity sensing in plants occurs in specialised tissues, like in the columella in root tips or the endodermis in shoots. Generally, dense organelles, acting as statoliths, are thought to interact with the cytosekeleton and ion channels in gravitropism. We examined the possibility that tobacco pollen tubes (Nicotiana sylvestris) having an elaborate cytoskeleton could perceive gravity through interaction of the cytoskeleton and the endomembrane system and organelles. Using lipid endocytosis as a quantitative parameter, we show that endocytosis is increased transiently in microgravity within 3 min. This increase is inhibited by the calcium blocker verapamil, suggesting that calcium is lowered in the tip, which is known to increase endocytosis in the pollen tube.
doi_str_mv	10.1111/plb.12061
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subjects	Boron Compounds - metabolism Endocytosis Endocytosis - drug effects Fluorescence Heterocyclic Compounds, 3-Ring - metabolism hypergravity microgravity Nicotiana - drug effects Nicotiana - growth & development Nicotiana - metabolism Nicotiana - physiology phospholipid Phospholipids - metabolism Pollen - drug effects Pollen - metabolism tobacco pollen Verapamil - pharmacology Weightlessness
title	Increased endocytosis of fluorescent phospholipid in tobacco pollen in microgravity and inhibition by verapamil
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