LC–MS/MS quantification of N-acetylneuraminic acid, N-glycolylneuraminic acid and ketodeoxynonulosonic acid levels in the urine and potential relationship with dietary sialic acid intake and disease in 3- to 5-year-old children

Red meat and dairy products contain high sialic acid (Sia) levels, but the metabolic fate and health impact in children remain unknown. The aims of the present study were to quantify the levels of urinary Sia N-acetylneuraminic acid (Neu5Ac), N-glycolylneuraminic acid (Neu5Gc) and ketodeoxynonuloson...

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Bibliographic Details
Published in:British journal of nutrition 2014-01, Vol.111 (2), p.332-341
Main Authors: Chen, Yue, Pan, Lili, Liu, Ni, Troy, Frederic A., Wang, Bing
Format: Article
Language:English
Subjects:
Acids
Animals
Biological and medical sciences
Body weight
Child
Child, Preschool
Children
Childrens health
China
Chromatography, Liquid
Dairy products
Diet
Eggs
Feeding. Feeding behavior
Female
Food Analysis
Fundamental and applied biological sciences. Psychology
Human and Clinical Nutrition
Humans
Male
Meat
Metabolism
Milk
N-Acetylneuraminic Acid - metabolism
N-Acetylneuraminic Acid - urine
Neuraminic Acids - urine
Nutrition
Sugar Acids - urine
Tandem Mass Spectrometry
Urine
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:Red meat and dairy products contain high sialic acid (Sia) levels, but the metabolic fate and health impact in children remain unknown. The aims of the present study were to quantify the levels of urinary Sia N-acetylneuraminic acid (Neu5Ac), N-glycolylneuraminic acid (Neu5Gc) and ketodeoxynonulosonic acid (KDN) and to determine their relationship with dietary Sia intake. Spot urine samples were collected from 386 healthy children aged 3 (n 108), 4 (n 144) and 5 (n 134) years at 06.30–07.00, 11.30–12.00 and 16.30–17.00 hours. Food intake levels were recorded on the day of urine sample collection. Sia levels were quantified using LC–MS/MS with [13C3]Sia as an internal standard. We found that (1) total urinary Sia levels in healthy pre-school children ranged from 40 to 79 mmol Sia/mol creatinine; (2) urinary Sia levels were independent of age and consisted of conjugated Neu5Ac (approximately 70·8 %), free Neu5Ac (approximately 21·3 %), conjugated KDN (approximately 4·2 %) and free KDN (approximately 3·7 %); Neu5Gc was detected in the urine of only one 4-year-old girl; (3) total urinary Sia levels were highest in the morning and declined over time in 4- and 5-year-old children (P
ISSN:0007-1145
1475-2662
DOI:10.1017/S0007114513002468