Addressing case specific biogas plant tasks: Industry oriented methane yields derived from 5L Automatic Methane Potential Test Systems in batch or semi-continuous tests using realistic inocula, substrate particle sizes and organic loading

•In-house upscale of Automatic Methane Potential Test System to 5L was developed.•Full-scale inocula, substrates and organic loading were used in tests.•Tests were characterized by short preparation times, accuracy and high-through put.•Same scales, equipment and methodologies were used in batch and...

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Bibliographic Details
Published in:Bioresource technology 2014-02, Vol.153, p.180-188
Main Authors: Kolbl, Sabina, Paloczi, Attila, Panjan, Jože, Stres, Blaž
Format: Article
Language:English
Subjects:
AMPTS II upscale
Anaerobic digestion
Automation
Batch
Batch Cell Culture Techniques - instrumentation
Batch Cell Culture Techniques - methods
Biofuels
Hydrolysis - drug effects
Industry
Methane - biosynthesis
Methane yield
Organic Chemicals - pharmacology
Particle Size
Semi-continuous
Time Factors
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Summary:•In-house upscale of Automatic Methane Potential Test System to 5L was developed.•Full-scale inocula, substrates and organic loading were used in tests.•Tests were characterized by short preparation times, accuracy and high-through put.•Same scales, equipment and methodologies were used in batch and continuous tests.•Increased full-scale decision making value of the developed approach was shown. The primary aim of the study was to develop and validate an in-house upscale of Automatic Methane Potential Test System II for studying real-time inocula and real-scale substrates in batch, codigestion and enzyme enhanced hydrolysis experiments, in addition to semi-continuous operation of the developed equipment and experiments testing inoculum functional quality. The successful upscale to 5L enabled comparison of different process configurations in shorter preparation times with acceptable accuracy and high-through put intended for industrial decision making. The adoption of the same scales, equipment and methodologies in batch and semi-continuous tests mirroring those at full scale biogas plants resulted in matching methane yields between the two laboratory tests and full-scale, confirming thus the increased decision making value of the approach for industrial operations.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2013.12.010