Hydrogel microspheres for stabilization of an antioxidant enzyme: Effect of emulsion cross-linking of a dual polysaccharide system on the protection of enzyme activity

•Development of catalase-loaded hydrogel microspheres.•Protecting the antioxidant enzyme against pH variation and proteolytic enzyme.•Scavenging intracellular H2O2 and protecting HT-29 cells. Catalase is an antioxidant enzyme abundant in natural resources. However, the enzyme is usually inactivated...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2014-01, Vol.113, p.59-68
Main Authors: Tang, Deh-Wei, Yu, Shu-Huei, Wu, Wen-Shin, Hsieh, Hao-Ying, Tsai, Yi-Chin, Mi, Fwu-Long
Format: Article
Language:English
Subjects:
Antioxidant enzyme
Antioxidants - chemistry
Antioxidants - metabolism
Catalase
Catalase - chemistry
Catalase - metabolism
Cell Survival - drug effects
Chitosan
Chitosan - analogs & derivatives
Chitosan - chemistry
Emulsions - chemistry
HT29 Cells
Humans
Hyaluronic Acid - chemistry
Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry
Hydrogen Peroxide - pharmacology
Microspheres
Polysaccharides - chemistry
Stabilization
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Summary:•Development of catalase-loaded hydrogel microspheres.•Protecting the antioxidant enzyme against pH variation and proteolytic enzyme.•Scavenging intracellular H2O2 and protecting HT-29 cells. Catalase is an antioxidant enzyme abundant in natural resources. However, the enzyme is usually inactivated by gastric acid and digestive enzymes after oral ingestion. In this study, carboxymethyl chitosan (CM-chitosan) and hyaluronic acid (HA) conjugate hydrogel microspheres have been prepared by an emulsion cross-linking technique to retain the activity of catalase in simulated gastrointestinal (GI) fluids. Cross-linking reduced the swelling capability and increased the resistance toward hyaluronidase digestion of prepared HA–CM-chitosan hydrogel microspheres. Catalase entrapped in the hydrogel microspheres exhibited superior stability over a wide pH range (pH 2.0 and 6.0–8.0) as compared to the native enzyme. The entrapped catalase was also protected against degradation by digestive enzymes. Following the treatments, the catalase-loaded microspheres, in contrast to native catalase, could effectively decrease the intracellular H2O2 level and protect HT-29 colonic epithelial cells against H2O2-induced oxidative damage to preserve cell viability. These results suggested that the HA–CM-chitosan hydrogel microspheres can be used for entrapment, protection and intestinal delivery of catalase for H2O2 scavenging.
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2013.09.002