LC-ESI-MS/MS analysis and pharmacokinetics of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma

ABSTRACT A simple, specific and reproducible liquid chromatography–electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was a...

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Bibliographic Details
Published in:Biomedical chromatography 2014-02, Vol.28 (2), p.193-196
Main Authors: Li, Yang, Liu, Xiao-Li, Cai, Zhen-Gang, Zhang, Si-Xi
Format: Article
Language:English
Subjects:
Animals
Antineoplastic Agents - blood
Antineoplastic Agents - chemistry
Antineoplastic Agents - pharmacokinetics
antitumor component
Chromatography, Liquid - methods
Diterpenes - blood
Diterpenes - chemistry
Diterpenes - pharmacokinetics
Drug Stability
Euphorbia - chemistry
jolkinolide B
LC-ESI-MS/MS
Least-Squares Analysis
Male
pharmacokinetics
Plant Extracts - chemistry
rat plasma
Rats
Rats, Wistar
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Summary:ABSTRACT A simple, specific and reproducible liquid chromatography–electrospray ionization mass spectrometry was developed and validated for the determination of jolkinolide B, a potential antitumor active component isolated from Euphorbia fischeriana, in rat plasma. Chromatographic separation was achieved on a Venusil MP‐C18 column using an isocratic elution. Jolkinolide B and osthole (internal standard) were monitored by positive electrospray ionization in the selected reaction monitoring mode. Good linearity (r2 > 0.996) was achieved by a weighted (1/x2) linear least‐squares regression over a concentration range of 6.50–2600 ng/mL. The accuracy and precision of the assay were satisfactory and the method proved to be applicable to pharmacokinetics following a single intravenous bolus injection of jolkinolide B to rats. Copyright © 2013 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3000