The impact of blood on liver metabolite profiling - a combined metabolomic and proteomic approach

ABSTRACT Metabolomics has entered the well‐established omic sciences as it is an indispensable information resource to achieve a global picture of biological systems. The aim of the present study was to estimate the influence of blood removal from mice liver as part of sample preparation for metabol...

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Bibliographic Details
Published in:Biomedical chromatography 2014-02, Vol.28 (2), p.231-240
Main Authors: Ly-Verdú, Saray, Schaefer, Alexander, Kahle, Melanie, Groeger, Thomas, Neschen, Susanne, Arteaga-Salas, Jose M., Ueffing, Marius, de Angelis, Martin Hrabe, Zimmermann, Ralf
Format: Article
Language:English
Subjects:
Animals
blood
Blood Chemical Analysis
Blood Proteins - analysis
Blood Proteins - isolation & purification
gas chromatography mass spectrometry
Gas Chromatography-Mass Spectrometry - methods
liquid chromatography tandem mass spectrometry
Liver - chemistry
Liver - metabolism
Male
Metabolome - physiology
metabolomics
Metabolomics - methods
Mice
mice liver perfusion
Mice, Inbred C3H
Multivariate Analysis
Principal Component Analysis
Proteome - analysis
proteomics
Proteomics - methods
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Summary:ABSTRACT Metabolomics has entered the well‐established omic sciences as it is an indispensable information resource to achieve a global picture of biological systems. The aim of the present study was to estimate the influence of blood removal from mice liver as part of sample preparation for metabolomic and proteomic studies. For this purpose, perfused mice liver tissue (i.e. with blood removed) and unperfused mice liver tissue (i.e. containing blood) were compared by two‐dimensional gas chromatography time of flight mass spectrometry (GC × GC‐TOFMS) for the metabolomic part, and by liquid chromatography tandem mass spectrometry (LC‐MS/MS) for the proteomic part. Our data showed significant differences between the unperfused and perfused liver tissue samples. Furthermore, we also observed an overlap of blood and tissue metabolite profiles in our data, suggesting that the perfusion of liver tissue prior to analysis is beneficial for an accurate metabolic profile of this organ. Copyright © 2013 John Wiley & Sons, Ltd.
ISSN:0269-3879
1099-0801
DOI:10.1002/bmc.3010