Isolation and characterization of restriction endonuclease EclHKI from an Enterobacter cloacae strain

An Enterobacter cloacae strain, producing restriction enzyme EclHKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam1105I, which recognizes and cleaves GACNNN!NNGTC. EclHKI was produced at high activity (4×10(4) U/g wet cells) and was purified from contaminants which interf...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 1994-01, Vol.10 (1), p.30-32
Main Authors: Chan, H Y, Chan, Y C, Kam, K M, Shaw, P C
Format: Article
Language:English
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Summary:An Enterobacter cloacae strain, producing restriction enzyme EclHKI, was isolated from a decaying potato. The enzyme is an isoschizomer of Eam1105I, which recognizes and cleaves GACNNN!NNGTC. EclHKI was produced at high activity (4×10(4) U/g wet cells) and was purified from contaminants which interfere with restriction digestion by passing the cell lysate through DEAE-Sephacel and heparin columns. Activity was optimal at 37°C in a medium salt buffer.
ISSN:0959-3993
1573-0972
DOI:10.1007/BF00357558