High Concentration of Gadolinium Ion Modifying Isolated Rice Mitochondrial Biogenesis

• Published in: Biological trace element research 2013-12, Vol.156 (1-3), p.308-315
• Main Authors: Zhao, Jie, Jin, Jian-Cheng, Zhou, Zhi-Qiang, Xia, Cai-Fen, Yang, Xiao-Gang, Jiang, Feng-Lei, Dai, Jie, Liu, Yi
• Format: Article
• Language: English
• Subjects: Agricultural biotechnology; Biochemistry; biogenesis; Biomedical and Life Sciences; Biotechnology; calcium; Calcium - metabolism; Calcium channels; Calcium Channels - metabolism; Cell Membrane - metabolism; Chelation; EDTA (chelating agent); endoplasmic reticulum; Endoplasmic Reticulum - metabolism; Gadolinium; Gadolinium - adverse effects; Gadolinium - pharmacology; growth and development; Life Sciences; membrane fluidity; membrane potential; Mitochondria; Mitochondria - metabolism; Mitochondrial Membrane Transport Proteins - metabolism; monitoring; Nutrition; Oncology; Oryza - metabolism; permeability; Plant growth; Plant Proteins - metabolism; plasma membrane; Rare earth elements; Rice; Studies; thiols; ultrastructure
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-013-9821-6

Mitochondria play an important role in plant growth and development, cooperating with the endoplasmic reticulum and nucleus. Gadolinium, one of the rare earth elements, is an inhibitor of stretch-activated calcium channels located on the endoplasmic reticulum and plasma membrane and has no effect on nuclear calcium variation in plant cells. We analyzed the effects of Gd³⁺ on mitochondria function by monitoring mitochondrial swelling, changes of membrane fluidity, and transmembrane potential collapse and by observing mitochondrial ultrastructure. We found that high concentration of Gd³⁺ induces rice mitochondrial dysfunction through mitochondrial permeability transition (MPT). The protection of DTT and EDTA demonstrate that Gd³⁺ blocks the inner membrane ion channel through thiol chelation.