Identification of a Plant Receptor for Extracellular ATP

Extracellular adenosine 5'-triphosphate (ATP) is an essential signaling molecule that is perceived in mammals by plasma membrane P2-type purinoceptors. Similar ATP receptors do not exist in plants, although extracellular ATP has been shown to play critical roles in plant growth, development, an...

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Published in:Science (American Association for the Advancement of Science) 2014-01, Vol.343 (6168), p.290-294
Main Authors: Choi, Jeongmin, Tanaka, Kiwamu, Cao, Yangrong, Qi, Yue, Qiu, Jing, Liang, Yan, Lee, Sang Yeol, Stacey, Gary
Format: Article
Language:English
Subjects:
Adenosine triphosphatase
Adenosine Triphosphate - metabolism
Animals
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Calcium
Enzyme Activation
Gene expression
Gene Expression Regulation, Plant
Genes
Kinases
Lectins
Mitogen-Activated Protein Kinases - metabolism
Mutation
Nucleotides
Plant cells
Plant growth
Plant resistance
Plasma
Protein Kinases - genetics
Protein Kinases - metabolism
Proteins
Receptors
Receptors, Mitogen - genetics
Receptors, Mitogen - metabolism
Receptors, Purinergic P2 - genetics
Receptors, Purinergic P2 - metabolism
Seedlings
Signal transduction
Stress, Physiological - genetics
Stress, Physiological - physiology
Up-Regulation
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Summary:Extracellular adenosine 5'-triphosphate (ATP) is an essential signaling molecule that is perceived in mammals by plasma membrane P2-type purinoceptors. Similar ATP receptors do not exist in plants, although extracellular ATP has been shown to play critical roles in plant growth, development, and stress responses. Here, we identify an ATP-insensitive Arabidopsis mutant, domi (Does not Respond to Nucleotides 1), defective in lectin receptor kinase 1.9 (Arabidopsis Information Resource accession code At5g60300). DORN1 binds ATP with high affinity (dissociation constant of 45.7 ± 3.1 nanomolar) and is required for ATP-induced calcium response, mitogen-activated protein kinase activation, and gene expression. Ectopie expression of DORN1 increased the plant response to physical wounding. We propose that DORN1 is essential for perception of extracellular ATP and likely plays a variety of roles in plant stress resistance.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.343.6168.290