The gastrin/cholecystokinin-B receptor on prostate cells – A novel target for bifunctional prostate cancer imaging

• Published in: European journal of pharmaceutical sciences 2014-02, Vol.52, p.69-76
• Main Authors: Sturzu, Alexander, Klose, Uwe, Sheikh, Sumbla, Echner, Hartmut, Kalbacher, Hubert, Deeg, Martin, Nägele, Thomas, Schwentner, Christian, Ernemann, Ulrike, Heckl, Stefan
• Format: Article
• Language: English
• Subjects: Animals; Bimodal contrast agent; Cell Line, Tumor; Contrast Media; Fluorescent Dyes - chemistry; Gadolinium; Gastrin receptor; Gastrins - chemistry; Heterocyclic Compounds, 1-Ring; Humans; Magnetic Resonance Imaging; Male; Mice; Mice, Nude; Oligopeptides - chemistry; Prostate - cytology; Prostate cancer; Prostatic Neoplasms - diagnosis; Prostatic Neoplasms - genetics; Receptor, Cholecystokinin B - genetics; Rhodamines - chemistry
• ISSN: 0928-0987; 1879-0720
• DOI: 10.1016/j.ejps.2013.10.013

In magnetic resonance imaging experiments with mice bearing human tumor xenografts the gastrin imaging conjugate shows clear preference of the PC3 prostate cancer tumor xenograft compared to native tissue and the Colo205 colon carcinoma xenograft. [Display omitted] The means of identifying prostate carcinoma and its metastases are limited. The contrast agents used in magnetic resonance imaging clinical diagnostics are not taken up into the tumor cells, but only accumulate in the interstitial space of the highly vasculated tumor. We examined the gastrin/cholecystokinin-B receptor as a possible target for prostate-specific detection using the C-terminal seven amino acid sequence of the gastrin peptide hormone. The correct sequence and a scrambled control sequence were coupled to the fluorescent dye rhodamine and the magnetic resonance imaging contrast agent gadolinium (Gd)-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA). Expression analysis of the gastrin receptor mRNA was performed by reverse transcriptase polymerase chain reaction on PC3 prostate carcinoma cells, U373 glioma, U2OS osteosarcoma and Colo205 colon carcinoma cells. After having confirmed elevated expression of gastrin receptor in PC3 cells and very low expression of the receptor in Colo205 cells, these two cell lines were used to create tumor xenografts on nude mice for in vivo experiments. Confocal lasers scanning microscopy and magnetic resonance imaging showed a high specificity of the correct conjugate for the PC3 xenografts. Staining of the PC3 xenografts was much weaker with the scrambled conjugate while the Colo205 xenografts showed no marked staining with any of the conjugates. In vitro experiments comparing the correct and scrambled conjugates on PC3 cells by magnetic resonance relaxometry and fluorescence-activated cell sorting confirmed markedly higher specificity of the correct conjugate. The investigations show that the gastrin receptor is a promising tumor cell surface target for future prostate-cancer-specific imaging applications.