Arabidopsis TOL Proteins Act as Gatekeepers for Vacuolar Sorting of PIN2 Plasma Membrane Protein

Controlling variations in plasma membrane (PM) protein abundance is of utmost importance for development in higher plants. For modulating PM protein activity, endocytosed proteins can be either cycled between PM and endosomes or sorted for their irreversible inactivation to lysosomes/vacuoles. Cargo...

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Bibliographic Details
Published in:Current biology 2013-12, Vol.23 (24), p.2500-2505
Main Authors: Korbei, Barbara, Moulinier-Anzola, Jeanette, De-Araujo, Lucinda, Lucyshyn, Doris, Retzer, Katarzyna, Khan, Muhammad A., Luschnig, Christian
Format: Article
Language:English
Subjects:
Arabidopsis - metabolism
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis Proteins - physiology
Protein Transport
Vacuoles - metabolism
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Summary:Controlling variations in plasma membrane (PM) protein abundance is of utmost importance for development in higher plants. For modulating PM protein activity, endocytosed proteins can be either cycled between PM and endosomes or sorted for their irreversible inactivation to lysosomes/vacuoles. Cargo ubiquitination triggers vacuolar delivery for degradation, which is controlled by Endosomal Sorting Complex Required for Transport (ESCRT) [1, 2]. Essential parts of this machinery are conserved across kingdoms, but determinants liable for initial recognition and concentration of ubiquitinated cargo have not been identified in plants [3, 4]. Here, we describe members of an Arabidopsis TOL (TOM1-LIKE) family as ubiquitin binding proteins that act redundantly in control of plant morphogenesis. Specifically, tol mutant combinations exhibit defects that reflect alterations in responses mediated by the phytohormone auxin. Consistently, we provide evidence for a role of TOLs in recognition and further endocytic sorting of a PIN-FORMED (PIN)-type auxin carrier protein at the PM, modulating dynamic auxin distribution and associated growth responses. Such TOL-dependent vacuolar sorting depends on cargo ubiquitination and coincides with dynamic rearrangements in TOL distribution. Collectively, these findings lead us to suggest a function for TOLs early in the passage of endocytosed ubiquitinated PM cargo, acting as gatekeepers for degradative protein sorting to the vacuole. •TOLs act early in the passage of endocytosed ubiquitinated PM cargo to the vacuole•TOLs functionally substitute for ESCRT-0 in higher plants•TOLs control auxin distribution and hormonal growth responses
ISSN:0960-9822
1879-0445
1879-0445
DOI:10.1016/j.cub.2013.10.036