Intragenic DNA methylation status down-regulates bovine IGF2 gene expression in different developmental stages

DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation....

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Bibliographic Details
Published in:Gene 2014-01, Vol.534 (2), p.356-361
Main Authors: Huang, Yong-Zhen, Zhan, Zhao-Yang, Sun, Yu-Jia, Cao, Xiu-Kai, Li, Ming-Xun, Wang, Jing, Lan, Xian-Yong, Lei, Chu-Zhao, Zhang, Chun-Lei, Chen, Hong
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Breeding
Cattle
DMR
DNA Methylation
Down-Regulation
Exons
Gene Expression
IGF2
Insulin-Like Growth Factor II - genetics
Male
Molecular Sequence Data
Muscle Development - genetics
qPCR
RNA, Messenger - genetics
Tissue Distribution
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Summary:DNA methylation is a key epigenetic modification in mammals and has an essential and important role in muscle development. Insulin-like growth factor 2 (IGF2) is a fetal growth and differentiation factor that plays an important role in muscle growth and in myoblast proliferation and differentiation. The aim of this study was to evaluate the expression of IGF2 and the methylation pattern on the differentially methylated region (DMR) of the last exon of IGF2 in six tissues with two different developmental stages. The DNA methylation pattern was compared using bisulfite sequencing polymerase chain reaction (BSP) and combined bisulfite restriction analysis (COBRA). The quantitative real-time PCR (qPCR) analysis indicated that IGF2 has a broad tissue distribution and the adult bovine group showed significant lower mRNA expression levels than that in the fetal bovine group (P
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2013.09.111