Short-term variability in biomarkers of bone metabolism in sheep

Changes in bone remodeling during pathological states and during their treatment can be assessed noninvasively by measuring biomarkers of bone metabolism. Their application is limited, however, by the potential biological variability in the levels of these biomarkers over time. To determine the shor...

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Bibliographic Details
Published in:Lab animal 2014-01, Vol.43 (1), p.21-26
Main Authors: Sousa, Cristina P., de Azevedo, Jorge T., Reis, Rui L., Gomes, Manuela E., Dias, Isabel R.
Format: Article
Language:English
Subjects:
Acid Phosphatase - blood
Alkaline Phosphatase - blood
Amino Acids - blood
Animal Models
Animals
Biological markers
Biomarkers - blood
Biomedical and Life Sciences
Bone and Bones - metabolism
Bone and Bones - physiology
Bone Remodeling - physiology
Bones
Calcium - blood
Identification and classification
Isoenzymes - blood
Life Sciences
Metabolism
Osteocalcin - blood
Peptide Fragments - blood
Phosphorus - blood
Physiological aspects
Portugal
Procollagen - blood
research-note
Sheep
Sheep - metabolism
Sheep - physiology
Statistics, Nonparametric
Tartrate-Resistant Acid Phosphatase
Time Factors
Veterinary Medicine/Veterinary Science
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Summary:Changes in bone remodeling during pathological states and during their treatment can be assessed noninvasively by measuring biomarkers of bone metabolism. Their application is limited, however, by the potential biological variability in the levels of these biomarkers over time. To determine the short-term variability in biomarkers of bone metabolism in adult sheep, the authors measured serum levels of alkaline phosphatase (ALP), bone-specific alkaline phosphatase (BALP), osteocalcin (OC), N-terminal propeptide of type-III procollagen (PIIINP), deoxypyridinoline (DPD), tartrate-resistant acid phosphatase (TRAP), calcium and phosphorus intermittently over a 12-week period. There were significant differences in mean ALP activity and in phosphorus concentrations over time, but all other biomarkers showed no significant short-term variability. The results suggest that biomarkers of bone metabolism in sheep, especially the bone resorption marker DPD and the bone formation marker BALP, can be used reliably to detect changes in bone cellular activity.
ISSN:0093-7355
1548-4475
DOI:10.1038/laban.418