Effect of culture medium type on canine adipose-derived mesenchymal stem cells and developmental competence of interspecies cloned embryos

Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate...

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Bibliographic Details
Published in:Theriogenology 2014-01, Vol.81 (2), p.243-249
Main Authors: Kim, Geon A., Oh, Hyun Ju, Lee, Tae Hee, Lee, Ji Hyun, Oh, Sang Hwan, Lee, Ju Hyun, Kim, Jin Wook, Kim, Se Woon, Lee, Byeong Chun
Format: Article
Language:English
Subjects:
Adipocytes - cytology
Animals
Canine adipose-derived mesenchymal stem cells
Cell Culture Techniques - veterinary
Cell Differentiation
Cells, Cultured
Cloning, Organism - methods
Cloning, Organism - veterinary
Culture Media
DMEM
Dogs
Embryonic Development
Gene expression
Gene Expression Profiling
iSCNT
iSCNT blastocyst
Mesenchymal Stromal Cells - cytology
Nuclear Transfer Techniques - veterinary
RCMEP
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Summary:Canine adipose-derived mesenchymal stem cells (ASCs) are promising as donor cells for somatic cell nuclear transfer (SCNT). It has been suggested that different cell cultures possess different capacities to support pre-implantation development of SCNT embryos. The aim of this study is to investigate whether two culture medium (RCMEP, Dulbecco's modified Eagle's medium [DMEM]) affect gene expression of ASCs, subsequent development of interspecies SCNT (iSCNT) and gene expression of cloned embryos. The RCMEP-cultured cells contained significantly greater amounts of SOX2, NANOG, OCT4, DNMT1, and MeCP2 than DMEM-cultured cells (P < 0.05). In iSCNT, the use of DMEM medium for culturing cells resulted in similar development to the blastocyst stage than those derived from RCMEP cultured cells (4.5% and 3.2%, respectively; P > 0.05). The expression of all transcripts except for DNMT1 in cloned blastocysts from RCMEP cultured cells followed those of cloned blastocysts derived from DMEM cultured cells. The alteration of gene expression in ASCs by culture medium was not manifested in the iSCNT embryos derived from these cells. Although the culture medium can induce changes of gene expression by ASCs, such alterations in donor cells did not affect the developmental competence or gene expression patterns of iSCNT embryos.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2013.09.018