transformation of protoplasts of Leptosphaeria maculans to hygromycin B resistance

Conditions are describe for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans . Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression s...

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Bibliographic Details
Published in:Current genetics 1988-04, Vol.13 (4), p.327-330
Main Authors: Farman, M.L, Oliver, R.P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Brassica
drug resistance
Fundamental and applied biological sciences. Psychology
Fungal plant pathogens
Genetic engineering
Genetic technics
genetic transformation
hygromycin B
Leptosphaeria maculans
Methods. Procedures. Technologies
pathogenicity
Phytopathology. Animal pests. Plant and forest protection
protoplasts
Variation, races, biotypes, parasitic specialization, genetics
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Summary:Conditions are describe for the efficient isolation and regeneration of protoplasts of a fungal pathogen of brassicas, Leptosphaeria maculans . Treatment of the protoplasts with DNA of the plasmid pAN7-1 (containing an E. coli hygromycin phosphotransferase gene with Aspergillus nidulans expression signals) and plating under selective conditions resulted in the formation of hygromycin B-resistant colonies. The demonstration of efficient transformation is an essential first step in the molecular analysis of pathogenicity of this commercially important pathogen.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00424427