Causes of retrograde flow in fish keratocytes

Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements...

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Published in:Cytoskeleton (Hoboken, N.J.) N.J.), 2014-01, Vol.71 (1), p.24-35
Main Authors: Fuhs, Thomas, Goegler, Michael, Brunner, Claudia A., Wolgemuth, Charles W., Kaes, Josef A.
Format: Article
Language:English
Subjects:
Actin Cytoskeleton - metabolism
actin network and myosin motors
Animals
atomic force microscopy
cell motility and forces
Cell Movement - physiology
Fishes
forces causing retrograde actin flow
Keratinocytes - cytology
Keratinocytes - metabolism
Microscopy, Atomic Force
Myosins - metabolism
polymerization and depolymerization force
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cited_by cdi_FETCH-LOGICAL-c4531-7d982cabc1dfa2a1c68447b02e00708320899e0c453ff08e73bb8301301fbe463
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container_title Cytoskeleton (Hoboken, N.J.)
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creator Fuhs, Thomas
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description Confronting motile cells with obstacles doubling as force sensors we tested the limits of the driving actin and myosin machinery. We could directly measure the force necessary to stop actin polymerization as well as the force present in the retrograde actin flow. Combined with detailed measurements of the retrograde flow velocity and specific manipulation of actin and myosin we found that actin polymerization and myosin contractility are not enough to explain the cells behavior. We show that ever‐present depolymerization forces, a direct entropic consequence of actin filament recycling, are sufficient to fill this gap, even under heavy loads. © 2013 Wiley Periodicals, Inc.
doi_str_mv 10.1002/cm.21151
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subjects Actin Cytoskeleton - metabolism
actin network and myosin motors
Animals
atomic force microscopy
cell motility and forces
Cell Movement - physiology
Fishes
forces causing retrograde actin flow
Keratinocytes - cytology
Keratinocytes - metabolism
Microscopy, Atomic Force
Myosins - metabolism
polymerization and depolymerization force
title Causes of retrograde flow in fish keratocytes
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