Immunohistochemical distribution of cell cycle proteins p27, p57, cyclin D3, PCNA and Ki67 in normal and diabetic human placentas

• Published in: Journal of molecular histology 2014-02, Vol.45 (1), p.21-34
• Main Authors: Unek, Gozde, Ozmen, Aslı, Mendilcioglu, Inanc, Simsek, Mehmet, Korgun, Emin Turkay
• Format: Article
• Language: English
• Subjects: Adult; Biomedical and Life Sciences; Biomedicine; Case-Control Studies; Cell Biology; Cyclin D3 - metabolism; Cyclin-Dependent Kinase Inhibitor p27 - metabolism; Cyclin-Dependent Kinase Inhibitor p57 - metabolism; Developmental Biology; Diabetes Mellitus - metabolism; Female; Humans; Immunohistochemistry; Ki-67 Antigen - metabolism; Life Sciences; Original Paper; Placenta - metabolism; Placenta - pathology; Pregnancy; Proliferating Cell Nuclear Antigen - metabolism; Young Adult
• ISSN: 1567-2379; 1567-2387
• DOI: 10.1007/s10735-013-9534-3

The placenta is a regulator organ for many metabolic activities between mother and fetus. Therefore, fetal growth is directly related to the placental development. Placental development is a series of events that depend on the coordinated action of trophoblasts’ proliferation, differentiation and invasion. Studies on cell cycle related proteins which control these events are fairly limited. How placental tissue proliferation is affected by diabetes is not exactly known yet. Therefore in this study, the immunohistochemical localizations of cell cycle related proteins like PCNA, Ki67, cyclin D3, p27 and p57 in the differentiation, proliferation and apoptosis mechanisms of normal and diabetic placentas were investigated. Information on cell cycle related proteins that control these events is limited and how they are affected in diabetes mellitus is not fully understood yet. Therefore, in this study, to understand the role of cell cycle regulators in diabetic placentas we aimed to determine the spatio-temporal immunolocalizations of cell cycle regulators in diabetic and normal human term placentas. Term placentas were obtained from diabetic women and from normal pregnancies with informed consent following caesarean deliveries. Placental samples were stained via immunohistochemistry with PCNA, Ki67, cyclin D3, p27 and p57 antibodies and were examined by light microscopy. When compared to control placentas, PCNA, Ki67 and cyclin D3 staining intensities significantly increased in villous parts of diabetes group. Moreover, Ki67 and cyclin D3 stainings also significantly increased in basal plates and chorionic plate respectively. In chorionic plates, p27 and p57 staining intensities significantly decreased in diabetic group. p57 staining also significantly decreased in villous parts of diabetic placentas. Placental abnormalities seen in diabetic placentas could be associated with proliferation and cell cycle arrest mechanisms’ alterations occurred in diabetes mellitus.