Functional characteristics of porcine peripheral T cells stimulated with IL-2 or IL-2 and PMA

In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swin...

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Bibliographic Details
Published in:Research in veterinary science 2014-02, Vol.96 (1), p.54-61
Main Authors: Cheon, In Su, Park, Sung-Moo, Lee, Hye Jin, Hong, Ji Eun, Ji, Sang Yun, Shim, Byoung-Shik, Kim, Kwang Ho, Heo, Pil Seung, Kim, Yoo Yong, Jung, Hyun Jung, Ka, Hakhyun, Han, Seung Hyun, Song, Manki, Yun, Cheol-Heui
Format: Article
Language:English
Subjects:
Adaptive Immunity - immunology
Animals
CD4+CD8+ double positive
CD4-Positive T-Lymphocytes - immunology
CD8-Positive T-Lymphocytes - immunology
Cell culture
Cell division
Cell Proliferation
Cloning
Cytokines - genetics
Cytokines - immunology
Flow Cytometry - veterinary
Hogs
Human
Humans
Immune system
Interleukin-2 - immunology
Leukocytes, Mononuclear
Lymphocyte Activation - immunology
Lymphocytes
Porcine
Reverse Transcriptase Polymerase Chain Reaction - veterinary
RNA, Messenger - chemistry
RNA, Messenger - genetics
Software
Swine - blood
Swine - immunology
T cells
Tetradecanoylphorbol Acetate - immunology
Veterinary medicine
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Summary:In human or mouse, mature T cells express either CD4 or CD8, resulting in different functions in the periphery. Interestingly, porcine CD4 and CD8 double positive (DP) T cells are present in the blood, and their proportions change from youth to adulthood. However, the features of these cells in swine are poorly understood. We investigated the fate of porcine peripheral T cells based on their functional characteristics, including proliferation and the expression of CD4 and CD8 co-receptors. The results showed that all the populations changed their CD8 expression in a time-dependent manner and porcine T cells had different proliferative pattern from human T cells. The results further revealed that Th2 cytokines were increased later in porcine T cells compared to human T cells upon stimulation with IL-2+PMA. Collectively, we found that the fate of porcine peripheral T cells is different from that of human T cells, and the changes occur in a time- and stimulation-dependent manner.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2013.11.018