Cloning and DNA sequence analysis of the cDNA for the precursor of the beta chain of bovine follicle stimulating hormone

Follicle stimulating hormone (FSH) plays essential roles in the maintenance and development of oocytes and spermatozoa in normal reproductive physiology. FSH possesses two subunits, α and β , the latter being responsible for FSH biological specificity. We have cloned and sequenced the cDNA encoding...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1986-09, Vol.83 (17), p.6618-6621
Main Authors: Esch, F.S, Mason, A.J, Cooksey, K, Mercado, M, Shimasaki, S
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Amino acids
Animals
Base Sequence
Biological and medical sciences
Biotechnology
BOVIDAE
BOVIDE
BOVIDO
Cattle
CLONACION
CLONAGE
CLONING
Cloning, Molecular
CODE GENETIQUE
CODIGO GENETICO
Complementary DNA
DNA
DNA - genetics
DNA probes
Follicle Stimulating Hormone - genetics
FSH
Fundamental and applied biological sciences. Psychology
Gels
Gene Expression Regulation
GENETIC CODE
Genetic engineering
Genetic technics
HFS
Hormones
Messenger RNA
Methods. Procedures. Technologies
Molecular cloning
Molecular Weight
Oligonucleotide probes
Pituitary Gland - physiology
RNA
Sheep
Ungulates
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Summary:Follicle stimulating hormone (FSH) plays essential roles in the maintenance and development of oocytes and spermatozoa in normal reproductive physiology. FSH possesses two subunits, α and β , the latter being responsible for FSH biological specificity. We have cloned and sequenced the cDNA encoding the FSH β chain from a bovine anterior pituitary cDNA library. The mature molecule is 109 amino acids long and is preceded by a 20-amino acid putative signal peptide. RNA gel blot analysis of bovine pituitary RNA shows that the mRNA encoding β chain of FSH is ≈ 1.7 kilobases in length.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.17.6618