Functional mapping of Autographa californica nuclear polyhedrosis virus genes required for late gene expression

A plasmid containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of an Autographa californica nuclear polyhedrosis virus (AcNPV) late gene promoter was constructed. This plasmid (pL2cat) also contained the AcNPV hr5 enhancer element. Transient-expression assay experi...

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Bibliographic Details
Published in:Journal of virology 1988-02, Vol.62 (2), p.463-471
Main Authors: Guarino, L.A, Summers, M.D
Format: Article
Language:English
Subjects:
ADN
AUTOGRAPHA
Autographa californica
Biological and medical sciences
DNA
Fundamental and applied biological sciences. Psychology
GENE
GENES
Genetics
INVERTEBRATE VIRUSES
Microbiology
nuclear polyhedrosis virus
PROTEIN SYNTHESIS
SINTESIS DE PROTEINAS
SPODOPTERA
SPODOPTERA FRUGIPERDA
SYNTHESE PROTEIQUE
Virology
VIRUS DE INVERTEBRADOS
VIRUS DES INVERTEBRES
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Summary:A plasmid containing the bacterial chloramphenicol acetyltransferase (CAT) gene under the control of an Autographa californica nuclear polyhedrosis virus (AcNPV) late gene promoter was constructed. This plasmid (pL2cat) also contained the AcNPV hr5 enhancer element. Transient-expression assay experiments indicated that the late promoter was active in Spodoptera frugiperda cells cotransfected with pL2cat and AcNPV DNA but not when pL2cat was transfected alone. Nuclease S1 mapping of infected and transfected RNAs indicated that the two open reading frames were transcribed as delayed-early genes. Quantitative nuclease S1 analysis and differential DNA digestion of recovered plasmids indicated that the activation of pL2cat was not due to an increase in steady-state levels of mRNA replication of the viral DNA.
ISSN:0022-538X
1098-5514
DOI:10.1128/JVI.62.2.463-471.1988