In vitro activities of antifungal drugs against dermatophytes isolated in Tokat, Turkey

• Published in: International journal of dermatology 2013-12, Vol.52 (12), p.1557-1560
• Main Authors: Yenişehirli, Gülgün, Tunçoğlu, Ebru, Yenişehirli, Aydan, Bulut, Yunus
• Format: Article
• Language: English
• Subjects: Amphotericin B - pharmacology; Antifungal Agents - pharmacology; Arthrodermataceae - drug effects; Arthrodermataceae - growth & development; Drug Resistance, Fungal; Epidermophyton - drug effects; Epidermophyton - growth & development; Epidermophyton floccosum; Griseofulvin - pharmacology; Humans; In Vitro Techniques; Itraconazole - pharmacology; Ketoconazole - pharmacology; Microbial Sensitivity Tests; Naphthalenes - pharmacology; Trichophyton - drug effects; Trichophyton - growth & development; Trichophyton mentagrophytes; Trichophyton rubrum; Trichophyton tonsurans; Trichophyton verrucosum; Turkey
• ISSN: 0011-9059; 1365-4632
• DOI: 10.1111/ijd.12100

Background In this study, we aimed to establish the in vitro antifungal susceptibilities of terbinafine, miconazole, itraconazole, ketoconazole, griseofulvin, and amphotericin B against dermatophyte isolates. Methods One hundred and seventy‐seven clinical isolates were tested: Trichophyton rubrum (n = 78), Trichophyton mentagrophytes (n = 49), Epidermophyton floccosum (n = 30), Trichophyton verrucosum (n = 16), and Trichophyton tonsurans (n = 4). The broth microdilution assay for antifungal susceptibility testing of dermatophytes was performed according to Clinical and Laboratory Standards Institute guidelines in the M38‐A2 document. Results Our minimum inhibitory concentration (MIC) results showed that the values for terbinafine for all dermatophyte isolates were significantly lower than the values for amphotericin B, miconazole, itraconazole, ketoconazole, and griseofulvin. For T. rubrum isolates, amphotericin B was more active than miconazole, itraconazole, and ketoconazole. Among the antifungal drugs tested, griseofulvin had the highest minimum inhibitory concentration values for T. mentagrophytes isolates. Conclusion Terbinafine was found to be the most effective antifungal drug against all tested dermatophyte isolates. Griseofulvin was the less active antifungal drug against T. mentagrophytes isolates. Performing antifungal susceptibility testing is especially important for screening the development of antifungal resistance.